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. 2010 Jan 14;285(13):10078–10086. doi: 10.1074/jbc.M109.034793

FIGURE 5.

FIGURE 5.

Met-75 cathepsin L and stefin B interact in the nucleus of living cells. In situ analysis of the interaction between stefin B-GFP and Met-75 cathepsin L was measured by FRET. A, visualization of Met-75 cathepsin L YFP and Arg-68 stefin B-GFP in CHO K1 cells. The plasmid constructs indicated on top of each column were transfected in CHO K1 cells. B, visualization of FRET and NFRET in CHO K1 cells pixel-by-pixel analysis of FRET on a cell expressing Met-75 cathepsin L-YFP + stefin B-GFP, performed with the PixFRET plug-in for the ImageJ software. C, NFRET in the nuclei of cells transfected with Met-75 cathepsin L YFP + stefin B compared with NFRET in cells transfected with expression vectors for Met-75 cathepsin L/Arg-68 stefin B-GFP. NFRET plot profiles in 25–30 cells were analyzed. Sensitized emission of YFP fusion proteins due to FRET was measured in two separate experiments in the nucleus of 25 individual CHO K1 cells transfected with the indicated combinations of vectors expressing YFP and GFP fusion proteins.