TABLE 2.
Intermolecular NOEs observed in the p140-(375–480)-DNA complex
| RFCp140-(375–480) | Ambiguously assigned to DNA | Unassigned intermolecular NOE | Experimentsa |
|---|---|---|---|
| ppm | |||
| Tyr-385 QD | CYT H5 or THY H1′ | 5.51 | A and B |
| CYT H6 or THY H6 | 7.72 | ||
| Asn-440 HD21 | CYT H5 or THY H1′ | 5.37 | A, B, and C |
| HB3 | TCH3 | 1.5 | A and B |
| Gly-416 HN | CYT H6, THY H6 | 7.7 | C |
| ADE H2, H8 or | |||
| NH2 of ADE, CYT and GUA | |||
| Arg-423 HE | Ribose H2″ or H2′ | 2.19 | B and C |
| Ribose H4′, H5″ or H5′ | 3.88 | ||
| Gly-455/Gln-456 HNa | Ribose H4′, H5″ or H5′ | 3.94 | A and B |
a A indicates two-dimensional F1-filtered [13C/15N] NOESY. B indicates two-dimensional NOESY. C indicates [15N,1H] NOESY-HSQC.
b Ambiguity in the amide proton resonance was due to overlap in amide proton frequencies between Gly-455 and Gln-456.