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Partial purification of the diol synthase activity of P. aeruginosa. A, gel filtration with TLC analysis of enzyme activity. The enzyme eluted between 15 and 17 min, suggesting a molecular size of ∼50 kDa based on the elution time of protein standards. B, ion exchange chromatography. The NaCl gradient started at 5 min, and the diol synthase activity eluted after 7.5 min (TLC analysis) at ∼0.3 m NaCl in the elution buffer.
