FIGURE 5.

Dependence of elastase production and vvpE transcription on IHF. Samples from the stationary phase cultures (A₆₀₀ = 2.0) of the wild type, smcR, and ihfA mutant were removed and analyzed for the elastase activity (A) and vvpE transcript (B). For a complementation test, when the cultures of the ihfA mutant containing pHS303 reached an A₆₀₀ of 0.6, IPTG was added to a final concentration of 0.1 mm to induce the expression of recombinant ihfA. WT, wild type; smcR, smcR mutant; ihfA, ihfA mutant. The error bars represent S.E.