FIGURE 6.

Activities of PL and PS in V. vulnificus with different genetic backgrounds. The vvpE promoter activities were determined separately by primer extension of the RNA derived from the wild type, ihfA mutant, and complemented strain, as indicated. Total RNA was prepared from the log phase (L; A₆₀₀ = 0.6) and stationary phase (S; A₆₀₀ = 2.0) of each culture. Lanes G, A, T, and C represent the nucleotide sequencing ladders of pKC980. The asterisks indicate the sites of the transcription starts for PS and PL, respectively. WT, wild type; ihfA, ihfA mutant.