TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Reference or source |
|---|---|---|
| Strains | ||
| V. vulnificus | ||
| ATCC29307 | Clinical isolate | Laboratory collection |
| HS03 | ATCC29307, smcR::nptI | 10 |
| HS05 | ATCC29307, ΔihfA | This study |
| E. coli | ||
| SM10 λpir | Thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu λpir, oriT of RP4, Kmr; conjugational donor | 26 |
| BL21(DE3) | F−, ompT, hsdS (rB−, mB−), gal(DE3) | Laboratory collection |
| Plasmids | ||
| pRK415 | Broad host range vector, IncP ori, oriT of RK2; Tcr | 34 |
| pDM4 | R6Kγ ori (requires π), oriT of RP4; Cmr | 43 |
| pHK0011 | pRK415 with promoterless luxAB; Tcr | 18 |
| pKC980 | pUC18 with vvpE; Apr | 32 |
| pHS201 | pHK0011 with 748-bp fragment of vvpE upstream region; Tcr | 10 |
| pHS206 | pH S201 with −206/−197substitution of vvpE upstream region; Tcr | This study |
| pHS176 | pH S201 with −176/−167substitution of vvpE upstream region; Tcr | This study |
| pHS136 | pH S201 with −136/−127substitution of vvpE upstream region; Tcr | This study |
| pHS106 | pH S201 with −106/−97substitution of vvpE upstream region; Tcr | This study |
| pHS56 | pH S201 with −56/−47substitution of vvpE upstream region; Tcr | This study |
| pHS221 | pH S201 with −101/−97deletion of vvpE upstream region; Tcr | This study |
| pHS222 | pH S201 with −106/−97deletion of vvpE upstream region; Tcr | This study |
| pHS301 | pGEM T with ihfA; Apr | This study |
| pHS302 | pGEM T with ΔihfA; Apr | This study |
| pHS3021 | pDM4 with ΔihfA; Cmr | This study |
| pHS303 | pRK415 with ihfA; Tcr | This study |
| pHS304 | pRSET C with ihfA; Apr | This study |
| pHS305 | pRSET C with ihfB; Apr | This study |
a Apr, ampicillin-resistant; Cmr, chloramphenicol-resistant; Kmr, kanamycin-resistant; Tcr, tetracycline-resistant.