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Identification of plasma proteins-specific mono-phages by ELISA. Different monoclonal phage culture supernatants were used as the first antibody against the plasma proteins and the negative control. The binding of each mono-phage was detected by anti-M13/HRP and 3,3′,5,5′-tetramethylbenzidine. KM13 helper phage was used as a negative binding control. The figure shows eight significantly positive mono-phage clones among hundreds of tests and one nonspecific clone F4.
