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. 2010 Jan 27;285(13):9402–9409. doi: 10.1074/jbc.M109.068205

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Wortmannin and dsRNA for PI4 kinase abolished the depolarization-induced increase of PIP₂ and potentiation of KCNQ2/Q3 currents. A, treatment with 10 μm wortmannin (WMN) for 10 min and 1 μm for 30 min reduced KCNQ2/Q3 currents. B, potentiation of KCNQ2/Q3 currents by depolarization (control, gray line) was mostly abolished by pretreatment with either 1 μm (dashed line) or 10 μm (solid line) wortmannin for 10 min. The initial current amplitudes immediately following depolarization to 0 mV were normalized. C, summary data for B. D, dsRNA for PI4Kβ abolished the ND96K-induced potentiation of KCNQ2/Q3 currents. **, p < 0.01. E, dsRNA for PI4Kβ abolished depolarization (0 mV)-induced increases of membrane PIP₂ level. F, Western blot of PI4Kβ for control, dsRNA-injected, ND96K-incubated, and dsRNA plus ND96K-incubated oocytes.