FIGURE 3.

Contribution of HDAC3 to TNF-α expression in LPS-stimulated cardiomyocytes. Cardiomyocytes were transfected with siRNA against HDAC1, -2, or -3 or control siRNA for 24 h and then exposed to LPS for 4 h. A–D, effects of HDAC3 siRNA. Transfection of HDAC3 siRNA down-regulated HDAC3 protein (A), decreased HDAC activity (B), and reduced TNF-α mRNA (C) and TNF-α protein (D). E and F, effects of HDAC1 and -2 siRNA. Knockdown of HDAC1 and -2 was confirmed by down-regulation of their mRNA levels (E1 and E2). The TNF-α mRNA levels were not altered by knockdown of HDAC1 or -2 (F). Data are mean ± S.D. (n = 3–5). *, p < 0.05 versus vehicle in saline or control siRNA; #, p < 0.05 versus vehicle in LPS.