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Loss of GAD65 affects apo- but not holo-GAD enzyme activity. GAD enzyme activity was measured in brain extracts of GAD65+/+ (n = 4, solid bars) and GAD65−/− (n = 4, open bars) mice in the presence or absence of PLP. Data were converted to pmol CO2 evolved per min/μg protein and expressed as percent of wild-type levels in the presence of PLP (2.69 ± 0.23 pmol per min/μg; mean ± SEM).
