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Specificity of the Hsp90 protective effect on tubulin. Shown is alteration of tubulin (16.3 μm) in the presence of geldanamycin (143 μm) (1), Hsp90 (20 μm) (2), Hsp90 and geldanamycin (20 and 143 μm, respectively) (3), and BSA (20 μm) (4). After incubation, samples were centrifuged, and supernatants (S) and pellets (P) were analyzed on an 8% SDS-PAGE. T, B, and H correspond to tubulin, BSA, and Hsp90, respectively. The presented experiment is representative of a set of 5.
