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. 2010 Jan 13;285(13):9583–9593. doi: 10.1074/jbc.M109.064956

FIGURE 1.

FIGURE 1.

SEQUENOM MassARRAY EpiTYPER analysis of DNMT1 and DNMT3L genes in human placenta and somatic tissues. A, placenta-specific methylation of the DNMT1 promoter assessed using the DNMT1_5 assay, spanning 230 bp from −133 to +97 relative to the transcription start site. Mean methylation in somatic tissues was 4.4% (across eight different tissues), whereas full term placental tissue shows a mean methylation level of 36.6% (n = 8 placentas). B, placenta-specific hypomethylation of the DNMT3L gene assessed using the DNMT3L_1 assay spanning 371 bp, −18/+353 relative to the transcription start site. Mean methylation in term placenta and purified cytotrophoblasts was 14 and 13%, respectively, whereas the mean for other tissues was 53% (n = 8) across three analyzable CpG units.