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. 2010 Jan 13;285(13):9583–9593. doi: 10.1074/jbc.M109.064956

FIGURE 3.

FIGURE 3.

Distribution of monoallelic methylation of the DNMT1 promoter in first trimester placental tissue and trophoblasts. Bisulfite sequencing data obtained using the DNMT1_1 assay in the following tissues: purified cytotrophoblasts (A) and uncultured (B) and cultured (C) (n = 10 passages) CVS biopsies. i, examples of data from individual samples; ii, mean methylation level for biological replicates (cytotrophoblasts, n = 2; uncultured and cultured CVS, n = 6). Shown are both sperm (D) and whole blood (E) lack of DNMT1 methylation as assessed by this methodology.