FIGURE 3.

HDAC4 suppresses MMP-13 gene expression. A, UMR 106-01 cells were transfected with 60 nm scrambled oligonucleotides or rat HDAC4 siRNA oligonucleotides. The cells were stimulated with PTH (10⁻⁸ m) for 30 and 240 min. Total RNA was extracted from the cells transfected with scrambled or siRNA oligonucleotides. RNAs were measured using Real time RT-PCR. The relative levels of mRNAs were normalized to β-actin and then expressed as fold stimulation over control. The error bars represent ± S.E. of three independent experiments. *, p < 0.05 versus scrambled oligonucleotides. B, the nuclear extracts from UMR 106-01 cells with or without PTH treatment were subjected to immunoblotting (IB) with anti-HDAC4 antibody. Anti-Cdk2 was used as a loading control.