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. 2010 Jan 29;285(13):9636–9641. doi: 10.1074/jbc.M109.062588

FIGURE 2.

FIGURE 2.

G9a and Glp methylate full-length p53 at Lys373. A, colloidal staining of in vitro methylation of p53 by G9a or Glp. Bovine serum albumin (BSA) serves as a negative control for enzymes, and H3 serves as a positive control for substrates. 2 μg of substrate and 1 μg of enzyme were used for each reaction. The components in each reaction are indicated. B, fluorography of in vitro methylation of p53 by G9a or Glp. A and B were identical volume aliquots from the same reaction.