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qRT-PCR analysis of the effects of Src transformation and contact normalization on the expression of Pdpn and Fhl1 mRNA. mRNA transcripts encoding Pdpn and Fhl1 from nontransformed cells, Src-transformed cells, and contact-normalized transformed cells were examined by qRT-PCR. Values were standardized to GAPDH as an internal control and are shown as expression intensities relative to Src-transformed cells that were normalized to a value of 1.0 (mean ± S.E. (error bars), n = 4).
