FIGURE 5.

Src utilizes Cas to augment Pdpn protein expression in transformed cells. a, protein from nontransformed and Src-transformed Cx43Ko cells, CasKo cells transfected with Cas or parental vectors, and wild type (WT) cells was analyzed by Western blotting to detect Pdpn, v-Src, active Src, Cas, and β-actin. b, Pdpn, Src, and nuclei were visualized by immunofluorescence microscopy in nontransformed and Src-transformed wild type and Cx43Ko cells. Differential interference contrast images are also shown. Scale bar, 100 μm.