FIGURE 1.

Exposure of mice to Cl₂ decreases alveolar fluid clearance. A, C57BL/6 mice were exposed to the indicated concentrations of Cl₂ gas for 30 min. Alveolar fluid clearance (expressed as % of instilled volume per 30 min) was measured 1 h post-exposure in anesthetized and ventilated mice as described under “Materials and Methods.” B, C57BL/6 mice exposed to 400 ppm Cl₂ for 30 min. Alveolar fluid clearance was measured 1 h post-exposure. Amiloride (Am.; 1.5 mm final concentration) or an equivalent volume of vehicle was added in the instilled solution in some animals. C, measurements of AFC in BALB/C mice exposed to 400 ppm Cl₂ and returned to room air for the indicated intervals. D, BALB/c mice were exposed to 400 ppm Cl₂ for 30 min. Alveolar fluid clearance was measured in the presence and absence of amiloride (AMI; mean ± S.E.; *, p < 0.05 compared with the corresponding air value; #, p < 0.05 compared with the corresponding value without amiloride).