FIGURE 13.
INa of βS520K mutant ENaC is not inhibited by HOCl. A, shown are representative time-course recordings of INa at −100 mV from oocytes expressing wild type α,βS520K and wild-type γ-ENaC perfused with HOCl-ND96 (1 mm) for 10 min (●) or incubated with HOCl-ND96 (100 μm) for 2 h (○), then perfused with trypsin (Try, 2 μm) and amiloride (Ami, 10 μm). B, group data showed neither perfusion nor incubation with HOCl-ND96 inhibits INa of βS520K mutant ENaC-expressing oocytes (mean ± S.E., n = 14 for HOCl perfused and 5 for HOCl incubated oocytes). Cont, control.