FIGURE 14.
Intratracheal trypsin reverses the decrease in AFC in mice exposed to Cl2. BALB/c mice were exposed to Cl2 (300 ppm for 30 min) and then returned to room air. Fifteen minutes post-exposure, they were briefly anesthetized with isoflurane, and trypsin (5 μm; dissolved in 100 μl of saline) was instilled dropwise in the nostrils. Mice recovered quickly, and 1 h post-exposure, they were anesthetized and ventilated, and AFC was measured (mean ± S.E.; control = 12; saline = 8; trypsin = 5. *, p < 0.05).