FIGURE 3.
HOCl decreases Na+ single channel activity in cell-attached patches of hENaC-expressing oocytes. Oocytes were patched in the cell-attached mode as described under “Materials and Methods,” currents were measured at a pipette holding potential of −100 mV (Vholding = Vapical − Ppipette), and amplitude distribution histograms were generated as described under “Materials and Methods.” Control (A) and 10-min post-perfusion with 2 mm HOCl-ND96 record (B) is shown. Open (O) and closed (C) states are indicated on the records. Notice a visible decrease of open state of single channels in HOCl-perfused oocytes. Typical records are from five control and five HOCl-perfused oocytes from two different batches. C, amplitude histograms for single channels from control (black) and HOCl perfused (red) oocytes are shown in A and B. D, NPo values were calculated from all amplitudes histograms as mentioned under “Materials and Methods” (mean ± S.E.; n = 5; **, p < 0.01).