TABLE 1.

Competition kinetics assessment of reactive centers in the 51-mer construct that are subject to chlorination by HOCl

Amino acida	kb	nc	n × k	Pd
Arginine	26	5	1.3 × 102	5.4 × 10−4
Asparagine	0.03	1	0.03	1.3 × 10−7
Cysteine	3.0 × 107	0	0	0
Glutamine	0.03	2	0.06	2.5 × 10−7
Histidine	1.0 × 105	2	2.0 × 105	0.83
Lysine	5.0 × 103	6	3.0 × 104	0.13
Methionine	3.8 × 107	0	0	0
Tryptophan	1.1 × 104	1	1.1 × 104	4.6 × 10−2
Tyrosine	44	0	0	0
Backbone amide	10	50	5 × 102	2.1 × 10−3

^a Only those amino acids with k ≥ 0.03 m⁻¹ s⁻¹ are listed here.

^b Rate constant (m⁻¹ s⁻¹) for the reaction of hypochlorite with the side chain of the amino acid or with the backbone amide nitrogen.

^c Number of this amino acid present in the 51-mer or number of backbone amide nitrogens in the 51-mer.

^d Normalized rate for reaction with HOCl calculated from the equation P = n_ik_i/(Σ_in_ik_i).