FIGURE 10.

Growth of cells expressing mutant actins. A, immunoblots of SDS-PAGE of total cell proteins of cells expressing FLAG-TEVCS-mutant actins. Upper gel, anti-FLAG antibody; lower gel, anti-actin antibody. B, growth curves of WT cells and cells expressing Y/Y, Y/F, Y/A, and Y/E actin. Cells expressing Y/A grew more slowly than Y/Y cells and to a lower maximum cell density. C, growth curves of cells expressing Y/Y, Y/L, and Y/W actin. Y/L and Y/W cells grew more slowly than Y/Y cells and to a lower maximum density. Because the plots are exponential, differences in growth rates and final cell densities appear to be less than they are (see “Results”).