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Properties of purified Y/W and Y/L actins compared with WT actin. A, SDS-PAGE of purified actins. B, nucleotide exchange of Y/W and Y/L actins is faster than for WT. C, Y/W polymerized at the same rate as WT, but Y/L polymerized more slowly. D, the critical concentrations of Y/W, Y/L, and WT are similar. E, Y/W and Y/L form filaments indistinguishable from filaments of WT. AU, arbitrary units.
