FIGURE 7.

Sympathetic neuron expression of dominant negative dynamin inhibits PACAP-stimulated Akt phosphorylation. A, control sympathetic neurons (CTL) and cultures infected with wild type (WT) or dominant negative dynamin K44A (DN) were extracted for Western analyses of dynamin protein expression. The two dynamin preparations yielded comparable levels of protein expression. B, sympathetic neuronal cultures infected with either dynamin (Dyn) WT or dynamin DN (48 h) were deprived of NGF for 4 h before the addition of 100 nm PACAP27 (4 h) for Western analyses of phosphorylated Akt levels. Cultures expressing dominant negative dynamin blocked PACAP-stimulated Akt activation. C, quantitative Western analyses of data in panel B were normalized to total culture Akt levels and expressed as -fold change from untreated control. n = 3, data represent the mean ± S.E. *, different from untreated controls at p < 0.05.