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Kinetic constants for 5′-3′ exonuclease activity of ttRecJ and cd-ttRecJ
| Protein | Kma | kcata | kcat/Km |
|---|---|---|---|
| μm | s−1 | m−1s−1 | |
| ttRecJ | 0.037 ± 0.0054 | 0.0047 ± 0.00025 | 130,000 ± 13,000 |
| cd-ttRecJ | 20 ± 2.8 | 0.0073 ± 0.00071 | 370 ± 14 |
a The assays were performed at pH 7.5 as described under “Experimental Procedures.” Km and kcat were determined by nonlinear regression analysis of the assay data.
