FIGURE 4.
The stability of Bcl-2 is specifically increased by its binding to FKBP38. Huh7 cells were transfected with a fixed amount of pEYFP-Bcl-2 (1 μg) and various amounts of pXJ-FLAG-FKBP38ΔNTD (A), pXJ-FKBP38ΔTPR (B) or pXJ-FKBP38ΔCBD (C) (0, 0.1, 0.3, and 0.8 μg). The cell lysates were analyzed by immunoblotting with anti-GFP and anti-FLAG antibodies. D and E, Huh7 cells were transfected with 1 μg of plasmid pECFP- Bcl-XL or 3 μg of plasmid pcMcl-1 either alone or together with 1 μg of the abovementioned FKBP38 truncation constructs. Total cell extracts were incubated with anti-Bcl-XL and -Mcl-1 antibodies and then analyzed by immunoblotting.