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. 2010 Jan 21;285(13):9792–9802. doi: 10.1074/jbc.M109.082305

FIGURE 2.

FIGURE 2.

Bay 11-7082 is a selective inhibitor of the NLRP3 inflammasome, whereas parthenolide can inhibit multiple inflammasomes. A and B, immunoblots of caspase-1 in culture supernatants of NG5 macrophages (A) and human THP-1 macrophages (B) treated with vehicle (DMSO), Bay 11-7082 (Bay; 12 μm), or parthenolide (Parth; 10 μm) for 15 min, followed by nigericin (Nig; 10 μm) for 45 min or MSU for 5 h as indicated. C and D, immunoblots of caspase-1 in culture supernatants of Nlrp3−/− macrophages treated with vehicle (DMSO), Bay 11-7082 (12 μm), or parthenolide (10 μm) for 15 min, followed by anthrax lethal toxin protective antigen/lethal factor (PA/LF) or Salmonella infection (Sal; multiplicity of infection of 20) for 3 h as indicated. Procasp1, procaspase-1.