FIGURE 1.

Effect of concentration of acceptor molecule on the incorporation of radioactivity from [¹⁴C]maltose-1-P into methanol-insoluble product. A, incubations contained 25 mm Tris-HCl buffer, pH 7.0, 125,000 cpm of maltose-1-P (12.5 nmol), various amounts of either mycobacterial glycogen (■), liver glycogen (▴), amylopectin (▾), or amylose (♦), and 25 μg of protein from the DE52 extract in a final volume of 300 μl. After an incubation of 20 min, reactions were stopped by placing tubes in a boiling water bath, and after cooling, 900 μl of methanol were added, and tubes were placed in a −20 °C freezer for 30 min. The precipitated glycogen was isolated by centrifugation, and its radioactive content was determined. B, incubations were as in A but contained various amounts of either mycobacterial glycogen (■) or liver glycogen (▴).