FIGURE 2.

Effect of concentration of maltose 1-phosphate on the incorporation of radioactive maltose into glycogen. Incubations contained 25 mm Tris-HCl buffer, pH 7.0, various amounts of maltose-1-P from 15 to 450 nmol (specific activity of 1000 cpm/nmol), 100 μg of oyster glycogen, and 25 μg of enzyme from DE52, all in a final volume of 0.3 ml. After an incubation of 20 min, glycogen was isolated as described in the text, and its radioactive content was determined. The data were also plotted by the method of Lineweaver and Burk as shown by the inset.