FIGURE 6.
Paper chromatographic identification of radioactive maltose resulting from arsenolysis of [14C]glycogen by GMPMT. GMPMT (50 μg) was incubated with 100,000 cpm of radioactive glycogen in the presence of 10 μmol of arsenate. Glycogen was precipitated with methanol, and the methanol supernatant liquid was taken to dryness, suspended in water, and applied to a column of DE52. The column was washed with water, and most of the radioactivity in the methanol supernatant fraction emerged in the water wash. This fraction was deionized with mixed-bed resin, and the radioactive sugar(s) was identified by paper chromatography as in Fig. 4. Standard sugars were as follows: maltotriose (TRI), maltose (MALT), and glucose (GLC).