TABLE 1.
Requirement for TreS and exogenous trehalose for accumulation of glycogen
Cells were grown in mineral salts medium with various amounts of trehalose as the carbon source. Cells were harvested after 40 h of growth and ruptured by sonication, and the glycogen was isolated from the cytoplasmic fraction. Cells are as follows: B11, wild type (WT); 47, trehalose-P-phosphatase negative mutant; 74, TreY negative mutant; 80, triple mutant missing trehalose-phosphate phosphatase; TreS, TreY, and unable to synthesize trehalose; mutant 91, missing TreS.
| Amount of trehalose in medium | Amount of glycogen in wild type and mutants |
||||
|---|---|---|---|---|---|
| WT (B11) | Mutant (47) | Mutant (74) | Mutant (80) | Mutant (91) | |
| % | nmol glucose/mg dry cells | ||||
| 0.1 | 15 | 31 | 13 | 15 | 15 |
| 2 | 341 | 188 | 315 | 4 | 13 |
| 4 | 514 | 370 | 317 | 7 | 11 |
| Presence of TreS in cells | + | + | + | − | − |