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Purification of GMPMT from M. smegmatis
| Purification steps | Activity | Specific activity | Purification |
|---|---|---|---|
| unitsa | units/mg protein | -fold | |
| Crude extract | 116,000 | 170 | 1 |
| Ammonium sulfate (35–75% fraction) | 85,000 | 290 | 2 |
| DE52 column (0.3 m NaCl) | 51,000 | 1,500 | 9 |
| Sephacryl-100 column | 15,000 | 15,200 | 89 |
a One unit of enzyme is defined as 1 nmol of maltose converted to glycogen in a 20-min incubation.
