TABLE 4.

Stoichiometry of GMPMT reaction

Incubations were as described in the text and contained 900 nmol of maltose-1-P (120,000 cpm). Incubations were for 15, 30, or 60 min, and the methanol supernatant was taken to dryness, suspended in water, and analyzed for its content of maltose-1-P remaining, as well as inorganic phosphate produced. The methanol precipitate was resuspended in water, and aliquots were taken for determination of radioactive content. Ratio of maltose incorporated into glycogen/inorganic phosphate produced was 1.0:0.89.

Incubation time	Radioactivity incorporated into glycogen		Amount of Malt-1-P used		Amount of inorganic phosphate appearing, nanomoles produced
	Total cpm	% total radioactivity added	Nanomoles disappearing	% of total maltose-P added
min
0	3400
15	25,000	21	150	16	200
30	42,000	35	300	33	250
60	67,000	56	600	66	450