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E2F1 and CDKN1C form stable complexes. A, MDA-MB-231 cells or HeLa cells were treated with 500 nm SNS-032 (48 h) or 100 nm dexamethasone (24 h). Western blotting confirms induction of endogenous CDKN1C and continued expression of E2F1. B, MDA-MB-231 cells or HeLa cells were treated as in A, and extracts were used in IP/Western blots using antibody to CDKN1C (or control IgG) for the IP step and E2F1 and CDKN1C antibodies for the Western step.
