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. 2010 Jan 29;285(13):9898–9907. doi: 10.1074/jbc.M109.059774

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Yeast two-hybrid analysis shows that specific Tectonin domains of GBP interact with CRP. Single, double, and triple GBP Tectonin domain subclones were tested for their interaction with CRP and with full-length GBP. The pGBKT7 vector containing GBP Tectonins domains/full-length GBP and pGADT7 vector containing CRP/full-length GBP were co-transformed and spotted on SD-Leu-Trp plates (double-dropout control) and SD-His-Ade-Leu-Trp (quadruple-dropout). The strength of interaction is indicated as +/−.