FIGURE 7.

Endogenous Wtip affects the rate of mouse podocyte spreading. Mouse podocytes stably expressing sh-Wtip or negative control sh-EMP vectors were plated onto collagen-coated coverslips and allowed to adhere and spread for the times indicated. A, sh-Wtip or sh-EMP cells were fixed and stained for Wtip (green), pan-cadherin (red), and nuclear dye TOPRO-3 (blue). Scale bars, 5 μm. B, histogram comparing the average cell diameter of sh-EMP and sh-Wtip cells after 24 h of reseeding onto collagen-coated coverslips. C, cells were fixed and stained with rhodamine-phalloidin and nuclear dye TOPRO-3 to visualize endogenous actin stress fibers and nuclei. Scale bars, 10 μm.