Figure 2.
Topical application of Tuhuai extract reduces the inflammatory response in both TPA and oxazolone models: ear inflammation was induced by topical TPA or oxazolone treatment. Skin samples were obtained 20 h after treatment. Significant reductions in inflammatory cell infiltration and ear thickness were seen in Tuhuai-treated ear. a and b are normal ear treated with Vehicle. c (TPA) and d (oxazolone) are inflammatory ear treated with Vehicle. e and f are inflammatory ear treated with Tuhuai. Scale bar = 100 μm. The ear thickness was measured with a digital caliper as described in Methods. Fig 2g is TPA model (P < 0.001 between all groups) and Fig 2h is oxazolone model (P < 0.01 between all groups). Data are expressed as mean + SEM. Numbers for each group are indicated in the figure.