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. 2010 Apr;176(4):1600–1606. doi: 10.2353/ajpath.2010.090406

Figure 2.

Figure 2

A: Proteins from control- (C) and AD-derived microvessel lysates were separated by SDS-PAGE, transferred to a polyvinylidene difluoride membrane, and immunoblotted for human thrombin (37 kDa). Loading equivalency was confirmed using GAPDH; n = 4. B: RNA from control (C) and AD-derived microvessels was extracted, reverse transcribed, and amplified using primers specific for human thrombin and the housekeeping gene GAPDH; n = 4. C: Proteins from control- (C) and AD-derived CSF were separated by SDS-PAGE, transferred to a polyvinylidene difluoride membrane, and immunoblotted for human thrombin (37 kDa). Loading equivalency was confirmed using albumin; n = 4.