Figure 4.

Association of luciferase activity with endogenous survivin expression in the CCl4-induced liver injury and repair in SPLucTg mouse model (Line 2). A 10% of CCl4 solution in corn oil at a dose of 200 μl/25 g was injected intraperitoneally on Day 0. Mice were sacrificed and livers were isolated on days 1, 2, 3, 4, and 5, respectively. After the isolated livers were lysed, luciferase activity (upper panel, mean of triplicate assays from same sample) and survivin expression (lower panel) were determined using luciferase activity assays and Western blot analysis, respectively. Examples of mice from each day were shown. HeLa cell lysates was used as a survivin positive control, and the molecular size of the protein markers in the protein marker lane was indicated. m indicates male mice; f, female mice.