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. 2010 Apr;176(4):1705–1715. doi: 10.2353/ajpath.2010.090451

Figure 3.

Figure 3

CXCR4 protein expression on myocardium after one week gene transfer and ischemia 30 minutes followed by 24 hours reperfusion. A: X-gal staining for β-gal expression, blue color is positive, (B) H-E staining, (C–D) immunostaining (DAB) for CXCR4 protein expression. C: CXCR4 group. D: β- gal control. E: CXCR4-overexpressed rats exhibit increased CXCR4 protein expression in ischemic myocardium after IR in vivo. Protein lysates were prepared from rats’ hearts one week after CXCR4 gene transfer followed by 30 minutes LAD ligation and 24 hours reperfusion. The ischemic and remote tissues were separated, lysed, and analyzed by Western blot. CXCR4 Mr; 40 to 47 kDa. Representative gel of three independent experiments is shown. Densitometric analysis of data from three different experiments is shown. *P < 0.05.