Figure 5.

CXCR3 regulates dermal-epidermal cross talk. A: Keratinocyte-fibroblast co-cultures were established in a Transwell system.4 Inserts with or without keratinocytes were treated with or without IFN-γ and/or EGF for 24 hours, and the fibroblast in the bottom wells were tested for motility by using a wound healing assay. Fibroblast cell motility was measured and showed that the motility of fibroblasts derived from CXCR3^−/− mice was not limited by the stimulation of keratinocytes by IFN-γ, unlike that of the wild-type fibroblasts. The addition of anti-CXCL11 antibody blocked the inhibitory effects of IP-9 on the wild-type mice fibroblast, whereas CXCR3^−/− fibroblast motility was unchanged. B: Fibroblasts alone were stimulated with recombinant CXCL11 with or without EGF, and tested for motility. EGF-induced motility of fibroblasts from the wild-type mice was blocked by the addition of CXC11, unlike that of CXCR3^−/− fibroblast motility. Shown is the mean ± SEM of n = 3, each in triplicate; *P < 0.05.