Figure 3.

Loss of mature tubular epithelial cell expression pattern after prolonged UUO. A: Decreased expression of EYFP and E-cadherin. Normal renal tubular epithelial cells in Cre^ksp;R26R-EYFP mice express EYFP in the cytoplasm (green) and E-cadherin at the basolateral aspect (red). With ureteral obstruction, tubular atrophy occurs as indicated by the decrease in EYFP-expressing cells. E-cadherin expression is down-regulated in the corresponding tubules. E-cdh, E-cadherin. B: Decreased expression of epithelial marker HNF-1β. HNF-1β expression (red) is decreased in EYFP-expressing epithelial cells (green) following UUO. Arrows indicate the nuclear expression of HNF-1β. Nuclei are counterstained with DAPI (blue). Scale bar = 20 μm.