Figure 2.

Activated hepatic Vα14iNKT cells promote apoptotic death of intrahepatic γδT cells after poly I:C treatment. Hepatic γδT cells were identified by FACS after cell surface staining with TCRγδ and CD3 mAbs. Hepatic γδT cells were fixed, permeabilized, and then stained intracellularly for active caspase 3. A representative FACS histogram (A) and absolute number (B) of intracellular active caspase 3-positive γδT cells are shown. Data are presented as means ± SEM with three to four mice per group from two independent experiments. *P ≤ 0.05 versus poly I:C-treated wild-type (WT) mice.