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. 2010 Apr;176(4):1779–1789. doi: 10.2353/ajpath.2010.090738

Figure 4.

Figure 4

Role of endogenous ROS in hepatic Vα14iNKT cell activation on poly I:C treatment. The ROS scavenger, NAC (300 mg/kg i.p.), was administered simultaneously with poly I:C in C57BL/6 wild-type (WT) mice or Jα18 KO mice. Control mice received vehicle PBS. A: Hepatic Vα14iNKT cells were identified by FACS using the CD1d tetramer and TCRβ mAb (see Materials and Methods) 16 hours after poly I:C treatment and then were stained for intracellular IFN-γ (as a marker for Vα14iNKT cell activation) after cell permeabilization. Results are presented as means ± SEM with four mice per group from two separate experiments. *P ≤ 0.05 versus vehicle. B: C57BL/6 wild-type mice and Jα18 KO mice were treated concurrently with NAC/poly I:C or PBS/poly I:C for intrahepatic γδT cell accumulation assessment by FACS 16 hours later. Data are presented as means ± SEM with four to six mice per group from two independent studies. ***P ≤ 0.05 versus all other groups; **P ≤ 0.05 versus vehicle-treated wild-type mice (no poly I:C) and poly I:C/PBS treated wild-type mice; *P ≤ 0.05 versus vehicle-treated wild-type mice (no poly I:C) and poly I:C/PBS-treated wild-type mice.