Figure 2.

Effect of antibodies on human umbilical vein endothelial tube formation assay and Matrigel plug assays: A: Human umbilical vein endothelial cells (20,000 per well) were premixed with test compounds at the final concentration of 10 μg/ml and plated on matrigel coated wells. Negative controls included PBS or unrelated mouse IgG. Positive control consisted of soluble EphB4 (sEphB4) applied at a concentration of 5 μg/ml (×20). Pictures were taken using a Bioquant Image Analysis system. B: Matrigel impregnated with test compounds (0.5 ml) was implanted in mouse abdominal wall. Plugs were harvested on day 7 and analyzed for vessel density (×10). Quantification of vessel density was done using image analysis system. Data are presented as mean ± SE.