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Preparation of Polyvalent RNA Gold Nanoparticle Conjugates. RNA duplexes (containing a double-stranded RNA sequence, an ethylene glycol spacer, and an alkylthiol group) are formed via hybridization and subsequently incubated with RNase free nanoparticles. Further passivation with oligoethylene glycol-thiol (OEG-Thiol) adds additional stability in cell culture.
