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. Author manuscript; available in PMC: 2010 Mar 22.
Published in final edited form as: Cancer Gene Ther. 2009 Feb 6;16(7):551–560. doi: 10.1038/cgt.2009.10

Figure 2.

Figure 2

Docetaxel does not promote G47Δ replication or enhanced cell-free virus in LNCaP cells. (a and b) Single-step growth curve assays demonstrate that pretreatment with a nontoxic range of docetaxel (0.025–0.2 nM) had minimal influence on G47Δ activity either at 24 (left panel) or at 48 h (right panel) after infection. (c) Cell-free virus assays reveal minimal influence of docetaxel pretreatment on G47Δ titers. LNCaP cells were pretreated with the indicated concentrations of docetaxel and 48 h after infection, supernatants and cells were collected separately, titered on Vero cells and the percentage of pfus in the supernatant was determined based on their combined pfus. (d) Micrographs of LNCaP cells pretreated with the indicated doses of docetaxel revealed negligible differences in X-gal staining 48 h after infection with G47Δ (multiplicity of infection; MOI 0.1).