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. Author manuscript; available in PMC: 2011 Mar 15.
Published in final edited form as: Chem Res Toxicol. 2010 Mar 15;23(3):696–704. doi: 10.1021/tx9004506

Figure 1.

Figure 1

Figure 1

Figure 1

Time Course of 3MI-Mediated Cytochrome P450 Induction in NHBE Cells. Quantitative real-time PCR analysis revealed maximal induction of CYP1A1 (A) transcript expression 4 hours following 10 µM 3MI exposure, whereas maximal induction of CYP2F1 (B) transcript expression occurred 1 hour after exposure. Values were standardized to Psmb6 transcript expression and are the means of 3 separate experiments * p < 0.05 compared to vehicle (DMSO)-treated control (white bar). Western blot analysis of CYP1A1 and CYP2F1 protein expression (C) after exposure to 10 µM 3MI at various time points revealed peak induction of protein expression at 4 hours, after which point protein levels decreased to initial levels. GAPDH was included as a loading control. Protein expression levels were the means of 3 separate experiments and were verified using densitometric analysis (D). * p < 0.05 compared to the levels at 0 hours.