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. 2010 Mar 3;30(9):3347–3357. doi: 10.1523/JNEUROSCI.5267-09.2010

Figure 1.

Figure 1.

Type 3 deiodinase expression in retinal development. A, Type 3 deiodinase activity in mouse eye homogenates. Activity was determined for pools of eyes at the ages indicated. Note that the x-axis scale differs at embryonic and postnatal ages. B, Northern blot detection of Dio3 mRNA in eye development. The main 2.2 kb Dio3 mRNA in eye was similar to that reported in the cochlea (Ng et al., 2009b). Brain and liver are positive and negative control tissues for Dio3 expression. 28S, rRNA control for RNA integrity detected by methylene blue staining. C, In situ hybridization detected Dio3 mRNA in all layers of the immature neural retina at E16.5 (top). Higher-magnification (bottom) shows signals for Dio3 at E18.5 and P2 and a control sense strand probe at P2. GCL, Ganglion cell layer; RPE, retinal pigmented epithelium. Scale bars: top, 50 μm; bottom, 20 μm. D, Loss of retinal type 3 deiodination in Dio3−/− mice. Plots show mean ± SD activity determined for individual pairs of eyes from 7 +/+, 21 +/−, and 12 −/− embryos at E18.5.